Highlights
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A serum preparation protocol for untargeted metabolomics using sequential NMR and multi-platform LC-MS analysis.
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No metabolite deuteration was observed when analysing samples in deuterated buffer using multiple LC-MS methods.
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LC-MS compound-feature abundances are minimally affected by NMR buffers and protein removal.
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A single serum sample preparation process for sequential NMR and multiple LC-MS platform analysis
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